Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name)
INTRODUCTION
Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months.
IgM antibodies are detectable within 5 days and persist for 2-3 months while IgG antibodies appear a few days later and provide a lifelong immunity.
PRINCIPLE
The dengue test employs colloidal gold particles coated with different recombinant dengue envelope proteins. When a specimen (serum) containing anti-dengue IgG and/or IgM antibodies is added to the test, the antibodies react with the dengue envelope proteins resulting in immune complexes which migrate along the membrane and are captured by monoclonal anti-human IgG in the first line and/or IgM in the second. The excess immune complexes then migrate further before binding to anti-dengue IgG which are coated on the control line.
If there are no dengue antibodies present in the specimen, only the control line will appear.
Note: The control line must appear in every test and the absence of the control line might indicates improper usage or the deterioration of the reagents.
Thank You
Stanley
0702201E
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Hi Stanley,
ReplyDeleteMay I know whether the results from this test is enough to make a diagnosis that the patient has dengue? Is there a need to do a follow up such as looking at the patient's blood sample under the microscope to comfirm the diagnosis? Thank you.
Zhu Zhijie Dennis
0700847G
TG01
Hey Stanley,
ReplyDeleteHow long does this test take? I suppose it's quite fast because some pregnancy kits uses the same mechanism too!
Yvonee
0703189A
Hi dennis,
ReplyDeleteIn the laboratory that i am doing my intership, we did not perform any other additional tests.
Hi Yvonee,
It takes 15mins for the test. Haha, its slightly longer than pregnancy kits which requires 3 or 5mins.
Thank You
Stanley
0702201E