I have learnt to build my own bubble trap, instead of buying pre-made ones available on the net. My bubble trap design looks something like the bottom diagram.
Principle:
The inlet will allow water to flow into the trap itself. With the air space in the vial, any bubble will will join with the air and be eliminated. Water will drop and exit via the outlet.
The filter has a pore size of 0.45um. It will block off most bacteria but not viruses. However, a filter of 0.45 pore size is enough for my experiment. This filter can be changed according to your preference.
Things needed:
1. Filter
2. Cryovial
3. Connectors
4. Forcep
5. Needle
Steps:
1. Using the needle, punch 3 holes on the cryovial. 1 on the top of the cap, 2nd at the bottom of the vial and 3rd at the side.
2. The holes wont be big enough for you to fit the connectors and filter in. Therefore, use a forcep to enlarge it manually. Which means, just dig and dig till the hole becomes big enough to fit.
3. Fit the filter and connectors and then the tubings and you get your own bubble trap.
Alvin
0703829B
14 July 2009
Hi Alvin
ReplyDeleteThat's simple
Would like to ask what type of experiment(s) are you doing that requires the bubble trap?
So for your experiment do u use the pre-made one or the "DIY" one. Would it be better to use the pre made one? Cause lets say the "DIY" may not be done correctly and then leads to some error.
Jordan Wong
TG02 Group 9
Hi alvin,
ReplyDeletewhat is the difference in the quality between your DIY and the pre-made one?
Do you only use 1 large cryovial or multiple cryovials stuck together, such that the solution will flow through multiple cryovials (or is that what you are doing already?)?
Lim Jia Hui
0703605F
TG01 Group 2
Hey Alvin,
ReplyDeleteCan I know what is the filter used for?
Thanks!
Siew Ming
0702862D
TG 01 Grp 2
What is the pore size of the filter? And what type of microorganisms it eliminates i.e. certain pore sizes will still allow certain microbes to pass through.
ReplyDeleteLi Yinliang Alex
TG02 0704894E
Group 8
Alvin,
ReplyDeleteYour post is poorly crafted. Your reader have no idea what you are doing and why you are doing this? This is not your personal diary and refrain from complaining. Your post will be disregarded if there is no improvement in the quality of your post.
Dr Lee
Jordan:
ReplyDelete1. I'm doing something regarding a bioreactor. The device is confidential so i cant say much.
2. I'm using the DIY one. Pre-made one will be better. Currently, my trap is leaking due to the difference in size of the hole created and the size of the connectors.
Joey:
1.Quality, pre-made one is better. The method of building it is quite crude. So there may be some problems here and there.
2. I'm using only 1 cryovial. Will post a photo of how my bubble trap looks like soon.
Siew Mai:
The filter is to filter off any microorganism, to prevent any contamination to the medium because when you connect the bubble trap to a device, medium will be flowing through it.
Alex:
1. 0.45um is the size for the filter pores.
2. It eliminates most bacteria but not viruses.
(I have added in the information in the post)
Dr Alex Lee:
I dont understand what you meant by readers dont understand what i'm writing. Is it the post or comment? Anyway, I change both. Hopefully it helps.
To all:
If there is still things that you dont understand, feel free to comment and i will clear your doubts to the best i can.
Alvin
hi alvin!
ReplyDeletecan this bubble trap be used on any device?
or is it designed specifically for a type of device?
stella
0701059H
Stella:
ReplyDeleteAS you can see, the size of this bubble trap it is quite small. So the device you use on cant be too big.
In addition, the device I'm using currently will be connected to small tubes, like those water hose you have at home. But instead of those big hose, the tubes I'm use is only 1.0mm in diameter.
Therefore, if the device that uses 1.0mm diameter tubes, this bubble trap can be use.
Alvin
19 July 2009
Hi Alvin,
ReplyDeleteSince there is a pre-made one, why are you making a DIY one that may cause a possibility that if it is not done well, there may be a contamination to the medium?
Also, what is a bioreactor? Can you explain a little on it so that we can have an idea of what it is. Thanks
Qingling
Qingling:
ReplyDeleteFor the premade ones, it comes with a standard volume. Meaning, it requires a certain amount of medium to run. For example, one of the premade one require at least 50ml of medium for it to work. Therefore, it is quite wasteful. In additon, my bioreactor do not work with so much medium.
A bioreactor is a device that help to create an physiological environment that is as close as the human body. (General term)
For example, on my experiment on the kidneys, the environment of our tubules cells are in constant motion rather than static. Therefore, if i use a normal culture flask, the medium wont be flowing. This is not how it happen in our kidney. Using a bioreactor, there is a flow. So its more or less quite similar to the kidney.
I cant tell you exactly how my bioreactor looks like. But it is something like a device that is connected to tubes, which will be then connected to a pump. This pump will allow the medium to flow. It is something like this.
Alvin
21 July 2009