CK-MB test

Creatine kinase (CK) is a dimeric enzyme which occurs in four different fours: a mitochondrial isoenzyme and CK-MM( muscle type), CK-BB (brain type) and CKMB.

The determination of CKMB is an important element in the diagnosis of myocardiac ischemia. CKMB is detectable in the blood about 3-8 hours after the onset of cardiac symptoms and can be detectable over a period of time which is dependant on the course of the condition.

Test principle

Sandwich principle

1. 1st incubation: 15uL of sample, a biotinlylated monoclonal anti CK-MB antibody, and a monoclonal CK-MB specific antibody labeled with a ruthenium complex react to form a sandwich complex.
2. 2nd incubation: After addition of streptavidin-coated microparticles, the complex becomes bound to the solid phase via interaction of biotin and streptavidin
3. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then washed away.
4. Results are determined bia a calibration curve which is instrument-specifically generated by 2 point calibration and a master curve provided via the reagent barcode.

Note: The entire test is done using the analyzer (Cobas e) and the test principle can be found inside the CK-MB kit.

Stanley