Dengue Test

Hello, my final blog posting will on the disposable Dengue test kit from HUMAN(Company name)

INTRODUCTION

Dengue is a flavivirus transmitted in the tropics and involves both humans abd Aedes mosquitos. The 4 serotypes of dengue (DEN-1, -2, -3 and -4) are similar, but are antigenically different. This implies that infection with one of the serotypes only provides cross-protective immunity for a few of months.



IgM antibodies are detectable within 5 days and persist for 2-3 months while IgG antibodies appear a few days later and provide a lifelong immunity.



PRINCIPLE

The dengue test employs colloidal gold particles coated with different recombinant dengue envelope proteins. When a specimen (serum) containing anti-dengue IgG and/or IgM antibodies is added to the test, the antibodies react with the dengue envelope proteins resulting in immune complexes which migrate along the membrane and are captured by monoclonal anti-human IgG in the first line and/or IgM in the second. The excess immune complexes then migrate further before binding to anti-dengue IgG which are coated on the control line.



If there are no dengue antibodies present in the specimen, only the control line will appear.



Note: The control line must appear in every test and the absence of the control line might indicates improper usage or the deterioration of the reagents.

Thank You

Stanley
0702201E